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· DOS Abstracts
Platelet-rich plasma leads to new matrix formation
around articular cartilage chips embedded in fibrin
glue in vitro
Morten Lykke Olesen, Natasja Leth Jørgensen, Bjørn Borsøe Christensen, Helle
Lysdahl, Martin Lind, Casper Bindzus Foldager
Orthopaedic Research Laboratory, Aarhus University Hospital; Orthopaedic
Research Laboratory, Aarhus University Hospital; Orthopaedic Research
Laboratory, Aarhus University Hospital; Orthopaedic Research Laboratory,
Aarhus University Hospital; Sports Trauma Clinic, Aarhus University Hospital;
Orthopaedic Research Laboratory, Aarhus University Hospital
Background:
Cellular outgrowth and formation of cartilaginous tissue around
articular cartilage explants has been described in a number of recent experi-
mental studies.
Purpose / Aim of Study:
We aimed to investigate the effect of platelet-rich
plasma (PRP) on new tissue formation around articular cartilage chips in vitro.
Materials and Methods:
Full thickness cartilage biopsies were isolated from
the femoral condyles of three skeletally mature Göttingen minipigs. The biop-
sies were prepared into 1mm3 cartilage chips. Cartilage chips were embed-
ded in fibrin glue and cultured in cell culture inserts up to 21 days in 1) control
media (Dulbecco’s Modified Eagle’s Media/F12, 10% fetal calf serum and 1%
penicillin- streptomycin), 2) control media with 10% autologous PRP or 3) 10%
autologous platelet- poor plasma (PPP) supplementation. Toluidine blue pH 4,
alcian blue pH 1 and hematoxylin & eosin (H&E) stainings were performed to
characterize newly formed matrix.
Findings / Results:
Cartilage chips were viable in all groups after 21 days of
tissue culturing. No pericellular clearing in H&E slides were observed. There were
no definite signs of chondrocytes from the chips in any of the groups. Histologic
evaluation revealed formation of negatively charged aggregates at the wound
edges of the cartilage chips in the PRP group compared with the control and
PPP groups. The majority of the cells found in these aggregates had a rounded
shape. The highly acidic alcian blue stain of the extracellular matrix indicated the
presence of glycosaminoglycans.
Conclusions:
The addition of PRP to fibrin glue-embedded cartilage explants
in vitro leads to formation of a glycosaminoclycan-rich and cell containing ag-
gregate surrounding the cartilage surfaces. This suggests a potential role of PRP
in new tissue formation when using cartilage explants embedded in fibrin glue.
No conflicts of interest reported
175.