DOS 2016

DOS Kongressen 2016 ·

Comparison of sheep adipose and bone marrow stem cells

on bone formation identified by anti-human vimentin

stain in an ectopic mouse model

Kristian Kjærgaard, Chris H Dreyer, Nicholas Ditzel, Christina M Andreasen, Li

Chen, Søren P Sheikh, Søren Overgaard, Ming Ding

Department of Orthopaedic Surgery and Traumatology, Odense University Hospital,

Institute of Clinical Research, University of Southern Denmark; Department of

Endocrinology and Metabolism, Molecular Endocrinology Laboratory (KMEB), Odense

University Hospital, University of Southern Denmark; Laboratory of Molecular and

Cellular Cardiology, Department of Clinical Biochemistry and Pharmacology, Odense

University Hospital.

Background:

Scaffolds for bone tissue engineering (BTE) can be loaded with mesen-

chymal stem cells (MSC) to improve osteogenesis. MSC can be found in bone marrow,

adipose tissue and other tissues. Little is known about osteogenic potential of adipose-

derived stem cells (ASC).

Purpose / Aim of Study:

This study aims to compare in vivo ostegenic capacity be-

tween ASC and bone marrow derived stem cells (BMSC).

Materials and Methods:

ASC and BMSC were isolated from five female sheep and

seeded on hydroxyapatite granules prior to subcutaneous implantation in 14 immuno-

deficient mice, four implants in each mouse. The doses of cells of the implants were

0.5×10^6, 1.0×10^6 and 1.5×10^6 ASC and 0.5×10^6 BMSC, respectively. After

eight weeks, bone volume vs. total volume was quantified using histomorphometry.

Origin of new bone was assessed using human vimentin (HVIM) antibody staining.

Findings / Results:

BMSC yielded significantly more bone than any ASC group, and dif-

ferences between ASC groups were not statistically significant. HVIM antibody stain was

successfully used to identify sheep cells in this model.

Conclusions:

ASC and BMSC were capable of forming bone. Though ASC were capable

of forming new bone as assessed by vimentin staining, in vitro treatment to enhance

osteogenic capacity is suggested as further research in ovine bone tissue engineering.

No conflicts of interest reported