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· DOS Abstracts
NO EFFECT OF PLATELET RICH PLASMA AS COAD-
JUVANT TO AUTOLOGOUS PARTICULATED CARTI-
LAGE FOR THE TREATMENT OF CHONDRAL DEFECTS
Morten Lykke Olesen, Bjørn Borsøe Christensen, Casper Bindzus Foldager, Kris
Chadwick Hede, Natasja Leth Jørgensen, Martin Lind
Orthopaedic Research Laboratory, Department of Orthopaedic Surgery, Aarhus
University Hospital;
Background:
Repair of chondral injuries by use of cartilage chips has recently
demonstrated clinical feasibility. Autologous platelet-rich plasma (PRP), may
offer promise in improving clinical outcomes as an adjunct to cartilage chips
treatment.
Purpose / Aim of Study:
To assess the histological quality of repair cartilage
tissue after autologous cartilage chips treatment (CC) with and without re-
peated local injections of PRP for the treatment of full-thickness focal chondral
defects of the knee.
Materials and Methods:
Two full-thickness chondral defects (Ø = 6 mm)
were surgically performed in the medial and lateral trochlea of each knee in six
skeletally mature Göttingen minipigs. The two treatment groups were 1) CC
with one weekly PRP injection for three weeks (n=12), and 2) CC alone (n=12).
The animals were euthanized after six months. Samples of both whole blood and
PRP were analysed with an automated hematology analyzer to determine the
concentrations of platelets and nucleated cells. The composition of cartilage re-
pair tissue was assessed using gross appearance as-sessment, histomorphom-
etry and semi-quantitative scoring (ICRS II).
Findings / Results:
The average fold increase in platelets was 6.8 ± 1.7. Leu-
kocyte concentration decreased in PRP samples by an average fold change of
1.9 ± 0.8. Histological evaluation demonstrated no significant difference in hya-
lin cartilage (CC+PRP: 18.7% vs. CC: 19.6%), fibrocartilage (CC+PRP: 48.1%
vs. CC: 51.8%) or fibrous tissue (CC+PRP: 22.7% vs. CC: 21.8%) between the
treatment groups.
Conclusions:
Four repeated local injections of leukocyte-reduced PRP after CC
in the treatment of full-thickness cartilage injuries demonstrated no beneficial
effects in terms of macroscopic and histological repair tissue quality.
No conflicts of interest reported
104.