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· DOS Abstracts
Characterization of the remodeling events contrib-
uting to trabecularization of cortical bone: A study
on human fibula diaphysis
Christina M. Andreasen, Jesper S. Thomsen, Lydia P. Bakalova, Annemarie Brüel, Ellen
M. Hauge, Gete E.T. Eschen, Birgitte J. Kiil, Jean-Marie Delaisse, Mariana E. Kersh,
Thomas L. Andersen
Orthopaedic Surgery & Traumatology, Odense University Hospital; Biomedicine,
Aarhus University; Mechanical Science & Engineering, University of Illinois at Urba-
na-Champaign, USA; Biomedicine, Aarhus University; Rheumatology, Aarhus Uni-
versity Hospital; Plastic Surgery, Aarhus University Hospital; Plastic Surgery, Aarhus
University Hospital; Clinical Cell Biology, Vejle Hospital/Lillebaelt Hospital; Mechani-
cal Science & Engineering, University of Illinois at Urbana-Champaign, USA; Clinical
Cell Biology, Vejle Hospital/Lillebaelt Hospital
Background:
The trabecularization of cortical bone leads to the fragilization of the
bones in elderly.
Purpose / Aim of Study:
To investigate the intracortical remodeling events con-
tributing to this endosteal trabecularization.
Materials and Methods:
Fibular diaphysis specimens from 19 patients (14 men, 5
women, 43–75 years) undergoing a jaw reconstruction. All specimens were plastic
embedded, µCT scanned and sectioned along the scanning plan, making it possible
to investigate the same canals analyzed in 3D by µCT and in 2D by histology.
Findings / Results:
The 3D analysis showed a 3.5-fold higher porosity and canals
with a 3-fold larger diameter at the endosteal half compared to the periosteal half.
The 2D analysis of these canals as intracortical pores showed that large pores (>100
µm diameter) were 3.3-fold more frequent in the endosteal half than in the perios-
teal half. A histological characterization of 948 pores revealed that these enlarged
pores are preferentially resorptive pores overlapping with the pore of a preexisting
parent osteon showing no signs of bone formation. The odds of being such a resorp-
tive pore were 1.6- fold higher in the endosteal half than in the periosteal half, and
6-fold higher in the enlarged pores (>100 µm diameter) than in the smaller pores.
The enlarged resorptive pores often resulted in coalescence of two or more pores.
The odds of finding these enlarged coalescent resorptive pores were 1.9- fold high-
er in the endosteal half than in the periosteal half, and 8-fold higher in the enlarged
pores (>100 µm diameter) than in the smaller pores.
Conclusions:
Both the 2D and 3D analyses showed that the cortical trabeculariza-
tion may in part result from the accumulation of enlarged resorptive pores/canals in
the endosteal part of cortex, suggesting that the bone formation is uncoupled from
the bone resorption in these pores.
No conflicts of interest reported
132.