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DOS Kongressen 2017 ·
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Regenerative tissue after matrix-associated cell
implantation has better quality using amplified
chondrocytes compared to synovial derived stem
cells in a rabbit model
Hagen Schmal, Anke Bernstein, Michael Seidenstücker, Katharina Böttiger, Eva
Johanna Kubosch
Department of Orthopaedics and Traumatology, Odense University Hospital;
Department of Orthopedics and Trauma Surgery, Albert-Ludwigs University
Medical Center Freiburg; Department of Orthopedics and Trauma Surgery, Al-
bert-Ludwigs University Medical Center Freiburg
Background:
The autologous chondrocyte implantation is an accepted method
to treat cartilage lesions. However, known problems regarding donor site mor-
bidity and the 2-step design make the search for cell alternatives ongoing.
Purpose / Aim of Study:
Aim of the study was to test the potential of SMSC
to regenerate cartilage using a matrix-associated implantation.
Materials and Methods:
SMSC were able to form cartilage in- vitro. In an
osteochondral defect model of the medial femoral condyle in rabbits, a collagen
type I/III membrane was seeded with either amplified allogenic chondrocytes
or SMSC and then transplanted into the lesion. A tailored piece synovial mem-
brane served as a control. Besides macroscopic and histological evaluation, the
regenerated tissue was examined biomechanically analyzing thickness, instant
and shear modulus.
Findings / Results:
The evaluation of the macroscopic degree of healing us-
ing the ICRS score and the area of healing did not show differences between
the groups after 6 weeks. Moreover, the thickness of the regenerated tissue
was higher in all intervention groups than in natural cartilage, but there was no
difference between the groups. However, the instant and shear modulus, re-
flecting the biomechanical strength, was superior in the implantation group us-
ing chondrocytes. Histologically, the regenerated lesions after matrix- coupled
chondrocyte implantation had a more chondrogenic structure and expressed
more proteoglycans, which was reflected by a lower Pritzker Score compared
to the controls. In the repair tissue of all groups the collagen types I, II, X were
expressed without statistical differences.
Conclusions:
Regenerated cartilage using undifferentiated SMSC for matrix-
associated implantation in a defect model in rabbits did not show a comparable
or higher quality than the current standard utilizing amplified chondrocytes.
No conflicts of interest reported
133.