DOS 2017

DOS Kongressen 2017 ·

PRP-enriched Allogenic Cartilage Decreases Risk of

Bone Bridge Formation after Physeal Injury in an Ex-

perimental Porcine Model

Ahmed Abdul-Hussein Abood, Bjarne Møller-Madsen, Juan Manuel Shiguetomi-

Medina, Morten Lykke Olesen, Hans Stødkilde-Jørgensen, Casper Bindzus Fold-

ager, Ole Rahbek

Children’s Orthopaedics, Aarhus University Hospital

Background:

The use of biological material is currently explored for preven-

tion of bone bridge formation, however no novel treatment technique has been

proposed. An improvement in current clinical practice can be of great value to

the affected children.

Purpose / Aim of Study:

To investigate the efficacy of allogenic cartilage

combined with platelet-rich plasma (PRP) for physeal repair in a porcine experi-

mental gap model.

Materials and Methods:

The study was carried out in six immature pigs. Al-

logenic cartilage was harvested from low-weight-bearing parts of the femoral

condyle in pigs from the same breed. The cartilage was frozen and stored. Pre-

operatively, autologous venous blood was drawn from the animal. It was centri-

fuged using a commercial PRP kit (GPS® III, Zimmer Biomet). Standardized cy-

lindrical defects were created in both hind legs of all included animals mimicking

a defect after resection of a physeal bone bridge. The right leg was randomized

for filling with allogenic cartilage, PRP and Tiseel® (Group A) or PRP and Tiseel®

(Group B). The left leg received the other treatment. The cartilage was thawed

and rinsed with saline. Perioperatively, it was cut into small chips of approxi-

mately 1 mm. The cartilage was blended with the Tiseel® and enriched with 1

mL of PRP. The mixture was inserted into the empty defect upon randomization.

The contralateral defect was filled with Tiseel® and enriched with 1 mL of PRP.

All animals underwent MRI scanning at 14 weeks.

Findings / Results:

Formation of a bone bridge occurred in one animal (17%)

in Group B. No animals (0%) formed a bone bridge in Group A. Water- content

MRI showed a mean of 16,7% higher water-content in Group A compared to B.

Conclusions:

Allogenic cartilage chips can prevent the formation of bone

bridges when combined PRP and Tiseel®. The use of allogenic cartilage can spare

the joint cartilage.

No conflicts of interest reported

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