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DOS Kongressen 2017 ·
101
Prevention of Bone Bridge Formation using Autologous
Cartilage in an Experimental Porcine Model
Ahmed Abdul-Hussein Abood, Bjarne Møller-Madsen, Juan Manuel Shiguetomi-
Medina, Hans Stødkilde-Jørgensen, Casper Bindzus Foldager, Ole Rahbek
Children’s Orthopaedics, Aarhus University Hospital; Children’s Orthopaedics,
Aarhus University Hospital; Orthopaedic Research Laboratory, Aarhus University
Hospital; MR Center Skejby, Aarhus University Hospital; Orthopaedics, Aarhus
University Hospital; Children’s Orthopaedics, Aarhus University Hospital
Background:
Bone bridges can occur due to physeal injury. This can lead to
partial growth arrest and bone deformities. The current treatment is ineffective.
Purpose / Aim of Study:
Investigate efficacy of autologous cartilage for phy-
seal repair in a porcine experimental gap model.
Materials and Methods:
Five immature pigs were included in the study. At
baseline the medial part of the distal femoral physis was injured in both legs us-
ing a 6 mm cannulated drill. The drill was inserted 1.5 cm into the bone creating
a standardized gap mimicking a defect after resection of a physeal bone bridge.
The defects were rinsed with sterile saline. Upon randomization, the right leg
was selected for either filling of defect with cartilage chips and Tiseel® (Group
A, n =5) or Tiseel only (Group B, n=5). Cartilage was harvested from low-
weight-bearing parts of the femoral condyle of the leg randomized for cartilage
treatment. A sharp incision through the skin, patellar ligament and Hoffa’s fat
pad was made. The articular surface was exposed. Through two 6 mm punches
sites were designated and harvested. The cartilage fragments were cut with a
scalpel perioperatively into smallest possible sized chips. The chips were molted
with the Tisseel® and inserted into the empty defect. Tiseel® was inserted into
the contralateral empty defect. The animals were housed for 14 weeks. MRI
was performed at 14 weeks.
Findings / Results:
No bone bridges were found on MRI in Group A. In Group B
one case of bone bridge formation was verified. The water- content measured
on MRI, showed a greater mean value (9,5%) in Group B.
Conclusions:
Bone bridges were prevented when autologous cartilage chips
were added to the Tiseel®. This suggests that transplantation of autologous
cartilage chips may play a role in preventing bone bridge formation.
No conflicts of interest reported
53.